Journal: Breast Cancer Research : BCR

Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

doi: 10.1186/s13058-025-02199-z

Figure Lengend Snippet: PAK2 expression and cluster formation in breast cancer cell lines. a mRNA expression levels of PAK2 in breast cancer cell lines and the normal mammary epithelial cell line MCF10A. b – f Quantification of cell cluster formation in four breast cancer cell lines under suspension culture conditions at 12, 24, and 48 h. g Immunoblot analysis of total PAK2 and phospho-PAK2 (Ser141) levels in BT549, MDA-MB-231, SKBR3, and AU565 breast cancer cells. SKBR3 cells were used as the reference (set to 1) for relative expression

Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

Techniques: Expressing, Suspension, Western Blot

Journal: Breast Cancer Research : BCR

Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

doi: 10.1186/s13058-025-02199-z

Figure Lengend Snippet: PAK2 promotes cell cluster formation. a , b Immunoblot analysis showing PAK2 expression in BT549 cells after overexpression or knockdown. c Representative images and quantification of cluster formation in BT549 cells transfected with control vector (Con) or lenti-PAK2 (OE-PAK2). d Representative images and quantification of cluster formation in BT549 cells transfected with control siRNA (siCon) or siPAK2-1

Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

Techniques: Western Blot, Expressing, Over Expression, Knockdown, Transfection, Control, Plasmid Preparation

Journal: Breast Cancer Research : BCR

Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

doi: 10.1186/s13058-025-02199-z

Figure Lengend Snippet: Effects of PAK2 on breast cancer cell progression. a , b Colony formation assays performed in BT549 cells with PAK2 overexpression or knockdown. c , d Wound healing assays evaluating the migration of BT549 cells with PAK2 overexpression or knockdown. e Transwell assays evaluating the invasive capacity of BT549 cells with PAK2 overexpression or knockdown

Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

Techniques: Over Expression, Knockdown, Migration

Journal: Breast Cancer Research : BCR

Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

doi: 10.1186/s13058-025-02199-z

Figure Lengend Snippet: PAK2 mediates cell cluster formation via E-cadherin phosphorylation. a , b GSEA showing the association between PAK2 and the adherens junction pathway. c , d Immunoblot analysis of E-cadherin and phosphorylated E-cadherin (Ser840) in BT549 cells after PAK2 overexpression or knockdown

Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

Techniques: Phospho-proteomics, Western Blot, Over Expression, Knockdown

Journal: Breast Cancer Research : BCR

Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

doi: 10.1186/s13058-025-02199-z

Figure Lengend Snippet: PAK2 interacts and colocalizes with E-cadherin. a Protein docking map and binding site of PAK2 for E-cadherin. The yellow color represents PAK2, and the blue color represents E-cadherin. b IF staining illustrating the colocalization of PAK2 and E-cadherin in MCF10A and BT549. c Co-IP analysis showing the association between PAK2 and E-cadherin in breast cancer cells

Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

Techniques: Binding Assay, Staining, Co-Immunoprecipitation Assay

Journal: Breast Cancer Research : BCR

Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

doi: 10.1186/s13058-025-02199-z

Figure Lengend Snippet: FRAX597 inhibits PAK2 activity and CTC cluster formation. a CCK-8 assay evaluating the viability of BT549 after 48 h treatment with FRAX597. b Immunoblot analysis of total PAK2 and phosphorylated PAK2 protein levels in BT549 treated with 2.5 or 5 µM FRAX597 for 24 and 48 h. c Representative images and quantification of BT549 cell aggregation after 24 h FRAX597 treatment. d Immunoblot analysis of total and phosphorylated PAK2, and total and phosphorylated E-cadherin in BT549 treated with 2.5 or 5 µM FRAX597 for 24 h

Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

Techniques: Activity Assay, CCK-8 Assay, Western Blot

Journal: Breast Cancer Research : BCR

Article Title: PAK2 promotes CTC cluster formation by phosphorylating E-cadherin to enhance cell-cell adhesion in breast cancer

doi: 10.1186/s13058-025-02199-z

Figure Lengend Snippet: FRAX597 suppresses breast cancer progression. a – c Effects of FRAX597 on BT549 cell proliferation, migration and invasion. d Schematic diagram of in vivo experimental design. e , f Tumor volume, tumor weight and body weight measurements of nude mice treated with FRAX597

Article Snippet: The human breast cancer cell lines BT549, MDA-MB-231, SKBR3, AU565, along with the normal mammary epithelial cell line MCF10A, were purchased from the American Type Culture Library (ATCC, Manassas, Virginia, USA).

Techniques: Migration, In Vivo